Picture | Name | English Name | Role |
| Anokata | That Person | Leader of the Black Organization. |
| Gin | Gin
Melkior (manga, later changed to Gin) | Member of the Black Organization. High ranking executive member. |
| Vodka | Vodka
Kaspar (manga, later changed to Vodka) | Member of the Black Organization. Often Gin's partner. |
| Vermouth | Sharon Vineyard
Chris Vineyard | Member of the Black Organization. She is an actress and master of disguise. Considered the Boss' "favorite," and knows that Haibara and Conan are really Sherry and Shinichi. |
| Chianti | Chianti | Member of the Black Organization. Considered an excellent sniper. |
| Korn | Korn | Member of the Black Organization. Considered an excellent sniper. |
| Bourbon | Bourbon | Member of the Black Organization. |
| Shiho Miyano | Shiho Miyano | Codenamed Sherry, she was a scientist for the Black Organization and creator of APTX 4869. Her sister's death caused her to betray the Black Organization. Her current alias is Ai Haibara. |
| Akemi Miyano | Akemi Miyano | Former member of the Black Organization, sister of Shiho Miyano, and the girlfriend of Shuichi Akai. She was killed by Gin for being a liability to the organization. |
| Elena Miyano | Elena Miyano | Shiho Miyano's mother. Deceased member of the Black Organization. |
| Atsushi Miyano | Atsushi Miyano | Shiho Miyano's father. Deceased member of the Black Organization. |
| Tequila | Tequila | Member of the Black Organization, originally trying to get programs and a list of the world's top programmers. Accidentally killed by a bomb. |
| Pisco | Pisco | An executive member of the Black Organization and a family friend of the Miyanos. His last assignment was to kill a politician. He was killed by Gin for getting caught in the act. |
| Calvados | Calvados | Member of the Black Organization, another sniper. Kills himself after Shuichi Akai disables him. He was in love with Vermouth. |
| Rikumichi Kusuda | Rikumichi Kusuda | Member of the Black Organization, who pretends to be a patient in the hospital holding Kir. He commits suicide when his cover is blown. |
| Ki'ichiro Numabuchi | Cornelius Graver | Serial killer whom Conan runs into a few times. Former member of the Black Organization, who scheduled to be the original test subject for APTX 4869 before he escaped. |